
One Screen
Nicotine Urine Cassette Test
COT 200
as low as $0.41 each
Case of 25
Discounted Pricing (Buy More & Save More)
Nicotine Urine Cassette Test – Case of 25 – OneScreen
The One Screen Nicotine Urine Cassette Test offers a fast, reliable way to detect cotinine — the primary metabolite of nicotine — in human urine. With a simple cassette format and built-in control, this test delivers accurate results in minutes. Whether used in clinics, workplaces, or personal settings, it’s a trusted tool for nicotine detection. Precision meets simplicity.
This test uses a cut-off level of 200 ng/mL, aligning with industry standards for cotinine detection. Results appear in just 5 minutes, making it ideal for high-volume screening environments. The cassette format allows for easy dropper-based sample application, with no need for additional reagents or equipment. A clear control line ensures test validity. It’s a one-step process designed for speed and clarity.
Each kit includes test cassettes, droppers, and instructions, packaged for convenience and hygiene. The test is disposable and single-use, reducing contamination risk and simplifying workflow. Whether you’re screening for tobacco use in insurance evaluations or monitoring cessation progress, this kit adapts to your needs. Compact and portable, it’s ready for field or office use.
Detect. Confirm. Quit.
Nicotine exposure can impact health outcomes, treatment plans, and workplace policies. This test helps verify tobacco use quickly and discreetly. It’s suitable for healthcare providers, employers, schools, and wellness programs. By identifying cotinine in urine, it supports informed decision-making and accountability. Early detection leads to better outcomes.
The One Screen Nicotine Test is for presumptive screening only — not for diagnostic or therapeutic decisions. A positive result indicates cotinine presence, but confirmatory lab testing may be required. It does not detect other drugs or substances. Always follow local testing protocols and privacy guidelines. Responsible screening starts with reliable tools.
Bulk orders are available for institutions, clinics, and corporate wellness programs. Whether you're conducting pre-employment screenings or supporting tobacco-free initiatives, this test delivers consistent performance. Order today to streamline your nicotine testing process. Because when it comes to health and compliance, speed and accuracy matter.
Features & Benefits:
-
High Sensitivity and Accuracy: Detects even trace amounts of target substances with precision, ensuring early detection and reliable screening. Trusted by professionals for its consistent performance across varied testing conditions.
-
Dual Detection & Rapid Results: Engineered for speed and clarity, the strips provide results in minutes with dual-line indicators—making it easy to interpret outcomes without delay or confusion.
- Portable and Compact: Each strip is individually sealed and lightweight, making it easy to carry, store, and distribute. Ideal for mobile testing units, field use, and on-the-go professionals.
-
Easy to Use & One-Step: No complicated setup or equipment required. Just dip, wait, and read—perfect for first-time users, busy clinics, and high-volume testing environments.
-
Built-In Control Line: Every strip includes a control line to verify the test is working properly. This built-in safeguard ensures accuracy and eliminates false negatives due to faulty strips.
-
Cost-Effective Solution: These test strips provide professional-grade testing at an affordable price point. Therefore, you get exceptional value without compromising on quality or accuracy.
-
Over 99% Accurate Results: Results appear in just minutes with industry-leading precision. As a result, you can make quick, informed decisions based on reliable data.
-
FREE Lab Confirmation Setup: For added peace of mind, we provide FREE lab setup to confirm positive results. Therefore, you gain extra verification and confidence in critical test outcomes.
-
Extended 24-Month Shelf Life: Each test strips remains effective for up to 24 months from the manufacturing date. As a result, they're ideal for bulk purchasing and dependable long-term storage.
-
FREE shipping on orders of 100 test strips or more.
-
In addition to this, bulk pricing structures allow you to save even more.
-
For inquiries or bulk purchases, call Cody at 318.798.3306.
All our drug test kits follow the SAMHSA Cutoff Levels.
These estimates serve as guidelines, yet the actual duration can vary widely based on factors such as the individual’s duration of drug use, dosage, and metabolism.
These are screening devices only and although very accurate if you get a positive we recommend a certified lab for LC-MS/MS Confirmation. For more information dial 1-866-526-2873, or email sales@americanscreeningcorp.com
Why Choose American Screening Corp
- U.S.-Based Manufacturer & Distributor: We offer an extensive range of urine test strips, from 1-panel to 5-panel options. As a result, you can find solutions tailored to your specific needs. In addition, our urine test strips are designed to ensure high performance.
-
Popular Options: These include 1, 2, and 5 test urine test strips. Consequently, customers can select precisely what suits their testing requirements. Moreover, these options provide flexibility for diverse environments.
-
High Quality: All urine test strips are made from durable, medical-grade plastic, ensuring reliability. Furthermore, they are specifically designed for professional use. Therefore, you can trust their consistent performance.
-
Easy to Use: A simple, one-step dip process delivers instant results. Because of this simplicity, minimal training is required. Additionally, users can confidently manage the testing process.
-
Regulatory Compliance: Adhering to all SAMHSA standards, thus ensuring results meet required guidelines. In addition, our compliance guarantees reliability and accuracy.
-
Customer Support: Our dedicated team provides expert advice and assistance throughout your purchase process. Moreover, they ensure you choose the right solution every time. As a result, you can enjoy a seamless experience.
-
Affordable Pricing: Bulk pricing and best-value offers help you save more. As such, the more you order, the greater your savings. Not only that, but special discounts ensure added affordability.
How to Use Nicotine Urine Cassette Test
Prepare the Test Kit: Bring the sealed cassette and dropper to room temperature (15–30°C). Open the pouch only when ready to test to maintain accuracy.
Collect the Urine Sample: Use a clean, dry container to collect a fresh urine specimen. Avoid contamination and test within 1 hour of collection for best results.
Add Sample to Cassette: Using the provided dropper, add 3–4 drops of urine into the sample well (marked “S”). Do not overfill or touch the test window.
Wait for Results: Lay the cassette flat on a clean surface. Wait 5 minutes before reading the results — do not interpret after 10 minutes.
Interpret the Results: Two lines = Negative (no cotinine detected), One line = Positive (cotinine present), No lines or no control line = Invalid — retest with a new cassette
Dispose Safely: Discard the used cassette and dropper in a biohazard container. Do not reuse any components.
FAQ – Nicotine Urine Cassette Test
1. What is Nicotine?
Nicotine is a highly addictive stimulant found in tobacco plants (Nicotiana tabacum). It is the primary psychoactive compound in cigarettes, cigars, chewing tobacco, and vaping products. Nicotine stimulates the central nervous system (CNS) and binds to nicotinic acetylcholine receptors, leading to the release of dopamine, which creates pleasurable effects and reinforces addiction.
2. What is the cut-off level?
The cut-off level for this test is 200 ng/mL. This means the test will detect Nicotine at or above this concentration in the urine sample.
3. How do urine strips work?
-
Dip the strip into a urine sample.
-
Wait for the specified time (usually 30 seconds to 2 minutes).
-
Compare the color changes on the strip to the reference chart provided.
4. How accurate are urine test strips?
High-quality test strips are 99% accurate when used correctly. However, accuracy can be affected by improper sample collection, contamination, or expired strips.
5. Can medications affect urine test results?
Yes, certain prescription medications, over-the-counter drugs, and even food items (like poppy seeds for opiates) may cause false positives. If unsure, confirm with a lab test.
6. Do urine test strips expire?
Yes, It have an expiration date and should be stored in a cool, dry place to maintain accuracy. Expired strips may give incorrect results.
7. Can urine strips detect pregnancy?
Yes, pregnancy test strips detect hCG (human chorionic gonadotropin) in urine, which indicates pregnancy.
8. Can test strips detect dehydration?
Yes, some strips measure specific gravity and ketone levels, which help indicate dehydration and electrolyte imbalances.
9. Can urine test strips be reused?
No, They are single-use only and should be discarded after testing.
10. How should I store It?
Store it in a cool, dry place away from moisture and direct sunlight. Keep them sealed in their original container to prevent contamination.
Version | SIze | ||
---|---|---|---|
Drug Test Specialty (IFU) | ML 12000.253 REV B | 1 MB | Download |
How It Works
Our 1 panel test strips are easy to use and the following step-by-step procedure helps you test in 3 easy steps.
1
Collect Sample
Add the required amount of urine sample into a clean container.

2
Apply Sample
Use the provided dropper to add a few drops of urine into the test cassette’s sample well.

3
Read Results
Wait for the indicated time (usually 5 minutes) and check the result lines for positive, negative, or invalid results.

How to read 1 panel test strips

POSITIVE
When only one colored band appears in the control region (C). No apparent colored band appears in the test region (T).

NEGATIVE
When two colored bands appear on the membrane and one band appears in the control region (C) and another band appears in the test region (T).
Certain lines may appear lighter or thinner than other lines.
Each line is to be considered a clear reading whether it be faint, light or dark.

INVALID
An invalid result is when a control band completely fails to appear.
Drug
- Alcohol
- Amphetamine
- Amphetamine
- Barbiturates
- Benzodiazepines
- Buprenorphine
- Cannabinoids
- Cocaine
- Cocaine
- Cotinine
- Ecstasy
- Ethylglucuronide
- Fentanyl
- Ketamine
- Kratom
- Lysergic Acid Diethylamide
- Methadone
- Methadone (EDDP)
- Methamphetamine
- Methamphetamine
- Methaqualone
- Methylphenidate
- Opiates
- Opiates (Morphine)
- Oxycodone
- Phencyclidine
- Propoxyphene
- Synthetic Cannabinoids
- Tramadol
- Tricyclic Antidepressants
Abbreviation
- ALC or ALCO
- AMP
- AMP300
- BAR
- BZO
- BUP
- THC
- COC
- COC150
- COT
- MDMA
- ETG
- FEN or FENT
- KET
- KRA
- LSD
- MTD
- EDDP
- MAMP or MET
- MAMP500 or MET500
- MQL
- MTHP
- OPI or OPl2000
- MOR or OPl300
- OXY
- PCP
- PPX
- K2
- TRA or TRAM
- TCA
Urine Cutoff· Level
- 20 mg/dl
- 1,000 ng/mL
- 300 ng/ml
- 300 ng/ml
- 300 ng/ml
- 10 ng/ml
- 50 ng/ml
- 300 ng/ml
- 150 ng/ml
- 200 ng/ml
- 500 ng/ml
- 500 ng/ml
- 10 ng/ml
- 1000 ng/ml
- 250 ng/ml
- 10 ng/ml
- 300 ng/ml
- 300 ng/ml
- 1,000 ng/mL
- 500 ng/ml
- 300 ng/ml
- 300 ng/ml
- 2,000 ng/mL
- 300 ng/ml
- 100 ng/ml
- 25 ng/ml
- 300 ng/ml
- 50 ng/ml
- 300 ng/ml
- 1,000 ng/mL
Saliva Cutoff· Level
- >0.02%B.A .C.
- 50 ng/ml
- 50 ng/ml
- 50 ng/ml
- 10 ng/ml
- 12 ng/ml
- 20 ng/ml
- 35 ng/ml
- 50 ng/ml
- 40 ng/ml
- 50 ng/ml
- 10 ng/ml
Approximate Detection Time in Urine
- Up to 24 Hours
- 2-4 Days
- 2-4 Days
- 4-7 Days
- 3-7 Days
- 1-4 Days
- 15-30 Days
- 2-4 Days
- 2-4 Days
- 2-3 Days
- 1-3 Days
- Up to 80 Hours
- 3-4 Days
- 7-14 Days
- 1-3 Days
- 2-5 Days
- 3-5 Days
- 3-5 Days
- 3-5 Days
- 3-5 Days
- Up to 6 Days
- 1-2 Days
- 2-4 Days
- 2-4 Days
- 2-4 Days
- 7-14 Days
- 1-2 Days
- Up to 3 Days
- 1-7 Days
- 7-10 Days
Approximate Detection Time in Saliva
- Up to 24 Hours
- 1-3 Days
- 1-3 Days
- 1-16 Hours
- 4-8 Hours
- 48-72 Hours
- 6-12 Hours
- 1-3 Days
- 1-3 Days
- 24-48 Hours
- 24-48 Hours
- Up to 80 Hours
- 24-48 Hours
- 24-48 Hours
- –
- 12-24 Hours
- 12-24 Hours
- 48-72 Hours
- 1-3 Days
- 1-3 Days
- 48-72 Hours
- 24-48 Hours
- 1-3 Days
- 1-3 Days
- 1-3 Days
- 1-3 Days
- 48-72 Hours
- 1-2 Days
- 48-72 Hours
Weight | 2 lbs |
---|---|
Dimensions | 4 × 5 × 3 in |
Adulterants |
No |
Drugs Tested For | COT |
Number of Drugs Tested | 1 |