One Screen
Nicotine Urine Cassette Test
PREDX-MDCT-U12 Case of 25
as low as $0.34 each
Quantities are individual tests, sold in full cases. Prices are per test.
Have a bulk order? Call (866) 526-2873 for best deals or request a quote →
Frequently bought together
Complete your collection setup with a recommended accessory.190 mL Plastic Beaker Cups with Temperature Strips
Recommended for your testing programProduct Details
Nicotine Urine Cassette Test – Case of 25 – OneScreen
The One Screen Nicotine Urine Cassette Test offers a fast, reliable way to detect cotinine — the primary metabolite of nicotine — in human urine. With a simple cassette format and built-in control, this test delivers accurate results in minutes. Whether used in clinics, workplaces, or personal settings, it’s a trusted tool for nicotine detection. Precision meets simplicity.
This test uses a cut-off level of 200 ng/mL, aligning with industry standards for cotinine detection. Results appear in just 5 minutes, making it ideal for high-volume screening environments. The cassette format allows for easy dropper-based sample application, with no need for additional reagents or equipment. A clear control line ensures test validity. It’s a one-step process designed for speed and clarity.
Each kit includes test cassettes, droppers, and instructions, packaged for convenience and hygiene. The test is disposable and single-use, reducing contamination risk and simplifying workflow. Whether you’re screening for tobacco use in insurance evaluations or monitoring cessation progress, this kit adapts to your needs. Compact and portable, it’s ready for field or office use.
Detect. Confirm. Quit.
Nicotine exposure can impact health outcomes, treatment plans, and workplace policies. This test helps verify tobacco use quickly and discreetly. It’s suitable for healthcare providers, employers, schools, and wellness programs. By identifying cotinine in urine, it supports informed decision-making and accountability. Early detection leads to better outcomes.
The One Screen Nicotine Test is for presumptive screening only — not for diagnostic or therapeutic decisions. A positive result indicates cotinine presence, but confirmatory lab testing may be required. It does not detect other drugs or substances. Always follow local testing protocols and privacy guidelines. Responsible screening starts with reliable tools.
Bulk orders are available for institutions, clinics, and corporate wellness programs. Whether you're conducting pre-employment screenings or supporting tobacco-free initiatives, this test delivers consistent performance. Order today to streamline your nicotine testing process. Because when it comes to health and compliance, speed and accuracy matter.
Features & Benefits:
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High Sensitivity and Accuracy: Detects even trace amounts of target substances with precision, ensuring early detection and reliable screening. Trusted by professionals for its consistent performance across varied testing conditions.
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Dual Detection & Rapid Results: Engineered for speed and clarity, the strips provide results in minutes with dual-line indicators—making it easy to interpret outcomes without delay or confusion.
- Portable and Compact: Each strip is individually sealed and lightweight, making it easy to carry, store, and distribute. Ideal for mobile testing units, field use, and on-the-go professionals.
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Easy to Use & One-Step: No complicated setup or equipment required. Just dip, wait, and read—perfect for first-time users, busy clinics, and high-volume testing environments.
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Built-In Control Line: Every strip includes a control line to verify the test is working properly. This built-in safeguard ensures accuracy and eliminates false negatives due to faulty strips.
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Cost-Effective Solution: These test strips provide professional-grade testing at an affordable price point. Therefore, you get exceptional value without compromising on quality or accuracy.
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Over 99% Accurate Results: Results appear in just minutes with industry-leading precision. As a result, you can make quick, informed decisions based on reliable data.
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FREE Lab Confirmation Setup: For added peace of mind, we provide FREE lab setup to confirm positive results. Therefore, you gain extra verification and confidence in critical test outcomes.
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Extended 24-Month Shelf Life: Each test strips remains effective for up to 24 months from the manufacturing date. As a result, they're ideal for bulk purchasing and dependable long-term storage.
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FREE shipping on orders of 100 test strips or more.
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In addition to this, bulk pricing structures allow you to save even more.
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For inquiries or bulk purchases, call Cody at 318.798.3306.
All our drug test kits follow the SAMHSA Cutoff Levels.
These estimates serve as guidelines, yet the actual duration can vary widely based on factors such as the individual’s duration of drug use, dosage, and metabolism.
These are screening devices only and although very accurate if you get a positive we recommend a certified lab for LC-MS/MS Confirmation. For more information dial 1-866-526-2873, or email sales@americanscreeningcorp.com
Why Choose American Screening Corp
- U.S.-Based Manufacturer & Distributor: We offer an extensive range of urine test strips, from 1-panel to 5-panel options. As a result, you can find solutions tailored to your specific needs. In addition, our urine test strips are designed to ensure high performance.
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Popular Options: These include 1, 2, and 5 test urine test strips. Consequently, customers can select precisely what suits their testing requirements. Moreover, these options provide flexibility for diverse environments.
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High Quality: All urine test strips are made from durable, medical-grade plastic, ensuring reliability. Furthermore, they are specifically designed for professional use. Therefore, you can trust their consistent performance.
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Easy to Use: A simple, one-step dip process delivers instant results. Because of this simplicity, minimal training is required. Additionally, users can confidently manage the testing process.
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Regulatory Compliance: Adhering to all SAMHSA standards, thus ensuring results meet required guidelines. In addition, our compliance guarantees reliability and accuracy.
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Customer Support: Our dedicated team provides expert advice and assistance throughout your purchase process. Moreover, they ensure you choose the right solution every time. As a result, you can enjoy a seamless experience.
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Affordable Pricing: Bulk pricing and best-value offers help you save more. As such, the more you order, the greater your savings. Not only that, but special discounts ensure added affordability.
How to Use Nicotine Urine Cassette Test
Prepare the Test Kit: Bring the sealed cassette and dropper to room temperature (15–30°C). Open the pouch only when ready to test to maintain accuracy.
Collect the Urine Sample: Use a clean, dry container to collect a fresh urine specimen. Avoid contamination and test within 1 hour of collection for best results.
Add Sample to Cassette: Using the provided dropper, add 3–4 drops of urine into the sample well (marked “S”). Do not overfill or touch the test window.
Wait for Results: Lay the cassette flat on a clean surface. Wait 5 minutes before reading the results — do not interpret after 10 minutes.
Interpret the Results: Two lines = Negative (no cotinine detected), One line = Positive (cotinine present), No lines or no control line = Invalid — retest with a new cassette
Dispose Safely: Discard the used cassette and dropper in a biohazard container. Do not reuse any components.
FAQ – Nicotine Urine Cassette Test
1. What is Nicotine?
Nicotine is a highly addictive stimulant found in tobacco plants (Nicotiana tabacum). It is the primary psychoactive compound in cigarettes, cigars, chewing tobacco, and vaping products. Nicotine stimulates the central nervous system (CNS) and binds to nicotinic acetylcholine receptors, leading to the release of dopamine, which creates pleasurable effects and reinforces addiction.
2. What is the cut-off level?
The cut-off level for this test is 200 ng/mL. This means the test will detect Nicotine at or above this concentration in the urine sample.
3. How do urine strips work?
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Dip the strip into a urine sample.
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Wait for the specified time (usually 30 seconds to 2 minutes).
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Compare the color changes on the strip to the reference chart provided.
4. How accurate are urine test strips?
High-quality test strips are 99% accurate when used correctly. However, accuracy can be affected by improper sample collection, contamination, or expired strips.
5. Can medications affect urine test results?
Yes, certain prescription medications, over-the-counter drugs, and even food items (like poppy seeds for opiates) may cause false positives. If unsure, confirm with a lab test.
6. Do urine test strips expire?
Yes, It have an expiration date and should be stored in a cool, dry place to maintain accuracy. Expired strips may give incorrect results.
7. Can urine strips detect pregnancy?
Yes, pregnancy test strips detect hCG (human chorionic gonadotropin) in urine, which indicates pregnancy.
8. Can test strips detect dehydration?
Yes, some strips measure specific gravity and ketone levels, which help indicate dehydration and electrolyte imbalances.
9. Can urine test strips be reused?
No, They are single-use only and should be discarded after testing.
10. How should I store It?
Store it in a cool, dry place away from moisture and direct sunlight. Keep them sealed in their original container to prevent contamination.
How It Works
| Version | SIze | ||
|---|---|---|---|
| Drug Test Specialty (IFU) | ML 12000.253 REV B | 1 MB | Download |
How It Works
Our 1 panel test strips are easy to use and the following step-by-step procedure helps you test in 3 easy steps.
1
Collect Sample
Add the required amount of urine sample into a clean container.

2
Apply Sample
Use the provided dropper to add a few drops of urine into the test cassette’s sample well.

3
Read Results
Wait for the indicated time (usually 5 minutes) and check the result lines for positive, negative, or invalid results.

How to read 1 panel test strips

POSITIVE
When only one colored band appears in the control region (C). No apparent colored band appears in the test region (T).

NEGATIVE
When two colored bands appear on the membrane and one band appears in the control region (C) and another band appears in the test region (T).
Certain lines may appear lighter or thinner than other lines.
Each line is to be considered a clear reading whether it be faint, light or dark.

INVALID
An invalid result is when a control band completely fails to appear.
Additional Information
| Weight | 2 lbs |
|---|---|
| Dimensions | 4 × 5 × 3 in |
| Adulterants |
No |
| Drugs Tested For | COT |
| Number of Drugs Tested | 1 |
| Box | CS25 |
Instructions & Documentation
Request the current Instructions for Use and lot-specific documentation from our team.
Shipping & Returns
Free shipping on orders over $299. Contact our team for delivery schedules on bulk orders. View the return policy →
Technical specifications
| Brand | One Screen |
|---|---|
| Product code | PREDX-MDCT-U12 |
| Specimen | Urine |
| Packaging | Case of 25 · CS25 |
| Size | 10 |
| Adulterants | No |
| Intended use | Professional use |
Urine Test Cassettes
The OneScreen Nicotine Urine Cassette Test is a reliable and easy-to-use solution for detecting nicotine metabolites in human urine at a 200 ng/mL cut-off level. Designed for individuals, work, and organizations, this compact and efficient test delivers quick and accurate results, helping to ensure a nicotine-free environment. With clear instructions and a user-friendly design, it provides a hassle-free testing experience, making it a trusted choice for routine or on-the-go nicotine screening.
Instructions for Use
Request the current manufacturer’s instructions before collection and interpretation.
Request One Screen IFU →Product documentation
Contact our team for the documents associated with this product and lot.
One Screen drug testing supplies
Explore One Screen products in a range of configurations. Ask our team about case quantities, product documentation and options for your screening program.
Shop One Screen products →Shipping & returns
Free shipping on orders over $299. Speak with our team about lead times and delivery schedules for bulk orders.
View return policy →Customer reviews
Read independently collected customer feedback about ASC products and service.
Read all customer reviews →YOUR TESTING WORKFLOW
From delivery to documentation.
Plan your workflow around the current product instructions and your program’s procedures.Receive
Check your shipment and product documentation.
Prepare
Review the current manufacturer’s Instructions for Use.
Collect
Collect the specimen using your program’s procedures.
Check
Review specimen temperature and validity indicators.
Read
Read and interpret results as directed by the IFU.
Document
Record results and follow your confirmation process.
WHY AMERICAN SCREENING
Support for every part of your program.
Product selection, case quantities and procurement support from a team that answers the phone.Documentation support
Request product instructions and lot-specific documentation.
Fulfillment support
Coordinate delivery schedules for recurring orders.
Bulk purchasing
Order case quantities for single-site or multi-site programs.
A team you can reach
Speak with our team about products and your next order.
Recurring supply
Plan repeat orders around your testing volume.
Procurement options
Ask about net terms and PO billing for qualified buyers.
SHOP BY INDUSTRY
Supplies for your testing program.
DOCUMENTATION
Every document your team needs.
Request current product and lot-specific documentation from our team.FREQUENTLY ASKED
Common questions from buyers.
How many tests are in one case?
This product is supplied in a case of 25. Select quantities in full cases.
Which substances does the panel screen for?
This test screens for the substances listed. Open Drugs Tested above for the full panel and cutoff table.
Can I request volume pricing or PO billing?
Yes. Contact our team with your monthly volume, panel requirements and delivery schedule. Net terms and PO billing are available for qualified buyers.
Where can I find product instructions?
Request the current Instructions for Use using the documentation links on this page.
What is the intended use of this product?
Follow the current manufacturer’s Instructions for Use and your program’s procedures.
Running a multi-site program?
Tell us your monthly volume, panel requirements and shipping schedule.
