

ASC
Cotinine Test Strips
COT 200
as low as $0.66 each
Case of 25
Discounted Pricing (Buy More & Save More)
Cotinine Test Strips – Case of 25
Nicotine exposure leaves behind cotinine—a stable metabolite that remains detectable long after the last puff. Cotinine Urine Test Strips are designed to identify recent tobacco or nicotine use in urine samples, offering a reliable screening tool for clinical, occupational, and forensic settings. Whether monitoring cessation, enforcing workplace policies, or supporting behavioral health, these strips deliver fast, accurate results. Discreet, dependable, and easy to use. Supporting informed decisions.
Each strip is calibrated to detect cotinine at a cut-off level of 200 ng/mL, balancing high sensitivity with minimal risk of false positives from environmental exposure. To use, dip the strip into a fresh urine sample for 10–15 seconds, lay it flat, and wait for results within five minutes. A control line confirms test validity. Two lines indicate a negative result; one line signals a positive. No lines? Retest with a new strip.
Cotinine can remain in the body for up to 3–4 days after nicotine intake, making this test ideal for post-event screening and abstinence verification. Widely used in rehab centers, probation programs, and wellness clinics, it offers a longer detection window than breath tests or saliva swabs. A trusted tool for nicotine monitoring and relapse prevention.
Detect. Monitor. Support.
Each strip is compact, individually sealed, and shelf-stable for 18–24 months. No refrigeration required. It fits seamlessly into clinical kits, outreach packs, or institutional workflows. Whether testing in the field or facility, it performs reliably with minimal training.
This product is intended for presumptive screening only. For diagnostic or legal confirmation, laboratory analysis is recommended. Suitable for forensic applications, cessation tracking, and wellness programs, it enables timely interventions and supports recovery strategies. Precision meets purpose.
Bulk packs available for clinics, schools, and outreach programs. Whether conducting routine screenings or distributing in high-risk zones, this test adapts to your operational needs. Trusted by healthcare teams, educators, and behavioral specialists. Order today to strengthen your response strategy. Because every test supports accountability. Action starts with insight.
Features & Benefits:
-
High Sensitivity and Accuracy: Detects even trace amounts of target substances with precision, ensuring early detection and reliable screening. Trusted by professionals for its consistent performance across varied testing conditions.
-
Dual Detection & Rapid Results: Engineered for speed and clarity, the strips provide results in minutes with dual-line indicators—making it easy to interpret outcomes without delay or confusion.
- Portable and Compact: Each strip is individually sealed and lightweight, making it easy to carry, store, and distribute. Ideal for mobile testing units, field use, and on-the-go professionals.
-
Easy to Use & One-Step: No complicated setup or equipment required. Just dip, wait, and read—perfect for first-time users, busy clinics, and high-volume testing environments.
-
Built-In Control Line: Every strip includes a control line to verify the test is working properly. This built-in safeguard ensures accuracy and eliminates false negatives due to faulty strips.
-
Cost-Effective Solution: These test strips provide professional-grade testing at an affordable price point. Therefore, you get exceptional value without compromising on quality or accuracy.
-
Over 99% Accurate Results: Results appear in just minutes with industry-leading precision. As a result, you can make quick, informed decisions based on reliable data.
-
FREE Lab Confirmation Setup: For added peace of mind, we provide FREE lab setup to confirm positive results. Therefore, you gain extra verification and confidence in critical test outcomes.
-
Extended 24-Month Shelf Life: Each test strips remains effective for up to 24 months from the manufacturing date. As a result, they're ideal for bulk purchasing and dependable long-term storage.
-
FREE shipping on orders of 100 test strips or more.
-
In addition to this, bulk pricing structures allow you to save even more.
-
For inquiries or bulk purchases, call Cody at 318.798.3306.
List of Cotinine Test Strips & Cut-off levels
Cotinine Test Strips | Urine Cutoff Levels | Detection Time |
COT200 (Cotinine) | 200 ng/mL | 1-3 Days |
These estimates serve as guidelines, yet the actual duration can vary widely based on factors such as the individual’s duration of drug use, dosage, and metabolism.
All our drug test kits follow the SAMHSA Cutoff Levels.
These estimates serve as guidelines, yet the actual duration can vary widely based on factors such as the individual’s duration of drug use, dosage, and metabolism.
These are screening devices only and although very accurate if you get a positive we recommend a certified lab for LC-MS/MS Confirmation. For more information dial 1-866-526-2873, or email sales@americanscreeningcorp.com
Why Choose American Screening Corp
- U.S.-Based Manufacturer & Distributor: We offer an extensive range of urine test strips, from 1-panel to 5-panel options. As a result, you can find solutions tailored to your specific needs. In addition, our urine test strips are designed to ensure high performance.
-
Popular Options: These include 1, 2, and 5 test urine test strips. Consequently, customers can select precisely what suits their testing requirements. Moreover, these options provide flexibility for diverse environments.
-
High Quality: All urine test strips are made from durable, medical-grade plastic, ensuring reliability. Furthermore, they are specifically designed for professional use. Therefore, you can trust their consistent performance.
-
Easy to Use: A simple, one-step dip process delivers instant results. Because of this simplicity, minimal training is required. Additionally, users can confidently manage the testing process.
-
Regulatory Compliance: Adhering to all SAMHSA standards, thus ensuring results meet required guidelines. In addition, our compliance guarantees reliability and accuracy.
-
Customer Support: Our dedicated team provides expert advice and assistance throughout your purchase process. Moreover, they ensure you choose the right solution every time. As a result, you can enjoy a seamless experience.
-
Affordable Pricing: Bulk pricing and best-value offers help you save more. As such, the more you order, the greater your savings. Not only that, but special discounts ensure added affordability.
How to Use Cotinine Test Strips
Prepare the Test Strip: Bring the sealed pouch to room temperature before opening. Remove the strip and use it promptly to ensure accuracy.
Collect the Urine Sample: Use a clean, dry container to collect a fresh urine specimen. Avoid contamination and ensure enough volume for dipping.
Dip the Strip: Hold the strip vertically with the arrow pointing down. Dip it into the urine for 10–15 seconds, below the MAX line.
Lay Flat & Wait: Remove the strip and place it on a non-absorbent flat surface. Wait for results to appear within 5 minutes.
Interpret the Results: Two lines = Negative (no COT detected), One line = Positive (COT present), No lines = Invalid — retest with a new strip
Dispose & Follow Up: Discard the used strip safely. For positive results, consult a healthcare or forensic professional for confirmation and next steps
FAQ – Cotinine Test Strips
1. What is cotinine and why is it tested?
Cotinine is a metabolite of nicotine. It remains in the body longer than nicotine itself, making it a reliable marker for recent tobacco or nicotine use.
2. How long after nicotine use can cotinine be detected in urine?
Cotinine can typically be detected in urine for up to 3–4 days after nicotine exposure, depending on usage frequency, metabolism, and hydration levels.
3. What is the cut-off level for Cotinine Urine Test Strips?
Most cotinine test strips are calibrated to a cut-off level of 200 ng/mL, balancing sensitivity with reduced risk of false positives from secondhand smoke or incidental exposure.
4. How do I use the test strip correctly?
Dip the strip into a fresh urine sample for 10–15 seconds, lay it flat, and wait for results within 5 minutes. A control line confirms the test is valid.
5. How do I interpret the results?
- Two lines: Negative (no cotinine detected above cut-off)
- One line: Positive (cotinine detected)
- No lines: Invalid test—repeat with a new strip
6. Can cotinine strips detect vaping or smokeless tobacco use?
Yes. Cotinine is produced from all nicotine sources, including cigarettes, e-cigarettes (vaping), nicotine patches, gum, and smokeless tobacco.
7. Are Cotinine Urine Test Strips suitable for home use?
Absolutely. They are designed for easy, at-home testing with clear instructions and fast results—ideal for personal monitoring or family use.
8. Do these strips require refrigeration or special storage?
No refrigeration is needed. Store them in a cool, dry place. They are shelf-stable for 18–24 months when sealed and stored properly.
9. Are the results legally binding or diagnostic?
No. These strips are intended for presumptive screening only. For legal or diagnostic confirmation, laboratory testing is recommended.
10. Who commonly uses cotinine test strips?
They are widely used by rehab centers, schools, probation programs, employers, wellness coaches, and individuals tracking nicotine cessation or exposure.
How It Works
Our 1 panel test strips are easy to use and the following step-by-step procedure helps you test in 3 easy steps.
1
Prepare Specimen
Put altleast 10 mg of your drugs in a clear dry container and add 5ml (1 teaspoon) of water for every 10 mg of your drugs and stir.

2
Testing
Dip the strip with the wavy side down in the water. Let the strip absorb the water for 15 seconds. Take the strip out and place it in a flat surface for 5 minutes.

3
Read Results
Read the results within 10 minutes, do not read results after 10 minutes.

How to read 1 panel test strips

POSITIVE
When only one colored band appears in the control region (C). No apparent colored band appears in the test region (T).

NEGATIVE
When two colored bands appear on the membrane and one band appears in the control region (C) and another band appears in the test region (T).
Certain lines may appear lighter or thinner than other lines.
Each line is to be considered a clear reading whether it be faint, light or dark.

INVALID
An invalid result is when a control band completely fails to appear.
Drug
- Alcohol
- Amphetamine
- Amphetamine
- Barbiturates
- Benzodiazepines
- Buprenorphine
- Cannabinoids
- Cocaine
- Cocaine
- Cotinine
- Ecstasy
- Ethylglucuronide
- Fentanyl
- Ketamine
- Kratom
- Lysergic Acid Diethylamide
- Methadone
- Methadone (EDDP)
- Methamphetamine
- Methamphetamine
- Methaqualone
- Methylphenidate
- Opiates
- Opiates (Morphine)
- Oxycodone
- Phencyclidine
- Propoxyphene
- Synthetic Cannabinoids
- Tramadol
- Tricyclic Antidepressants
Abbreviation
- ALC or ALCO
- AMP
- AMP300
- BAR
- BZO
- BUP
- THC
- COC
- COC150
- COT
- MDMA
- ETG
- FEN or FENT
- KET
- KRA
- LSD
- MTD
- EDDP
- MAMP or MET
- MAMP500 or MET500
- MQL
- MTHP
- OPI or OPl2000
- MOR or OPl300
- OXY
- PCP
- PPX
- K2
- TRA or TRAM
- TCA
Urine Cutoff· Level
- 20 mg/dl
- 1,000 ng/mL
- 300 ng/ml
- 300 ng/ml
- 300 ng/ml
- 10 ng/ml
- 50 ng/ml
- 300 ng/ml
- 150 ng/ml
- 200 ng/ml
- 500 ng/ml
- 500 ng/ml
- 10 ng/ml
- 1000 ng/ml
- 250 ng/ml
- 10 ng/ml
- 300 ng/ml
- 300 ng/ml
- 1,000 ng/mL
- 500 ng/ml
- 300 ng/ml
- 300 ng/ml
- 2,000 ng/mL
- 300 ng/ml
- 100 ng/ml
- 25 ng/ml
- 300 ng/ml
- 50 ng/ml
- 300 ng/ml
- 1,000 ng/mL
Saliva Cutoff· Level
- >0.02%B.A .C.
- 50 ng/ml
- 50 ng/ml
- 50 ng/ml
- 10 ng/ml
- 12 ng/ml
- 20 ng/ml
- 35 ng/ml
- 50 ng/ml
- 40 ng/ml
- 50 ng/ml
- 10 ng/ml
Approximate Detection Time in Urine
- Up to 24 Hours
- 2-4 Days
- 2-4 Days
- 4-7 Days
- 3-7 Days
- 1-4 Days
- 15-30 Days
- 2-4 Days
- 2-4 Days
- 2-3 Days
- 1-3 Days
- Up to 80 Hours
- 3-4 Days
- 7-14 Days
- 1-3 Days
- 2-5 Days
- 3-5 Days
- 3-5 Days
- 3-5 Days
- 3-5 Days
- Up to 6 Days
- 1-2 Days
- 2-4 Days
- 2-4 Days
- 2-4 Days
- 7-14 Days
- 1-2 Days
- Up to 3 Days
- 1-7 Days
- 7-10 Days
Approximate Detection Time in Saliva
- Up to 24 Hours
- 1-3 Days
- 1-3 Days
- 1-16 Hours
- 4-8 Hours
- 48-72 Hours
- 6-12 Hours
- 1-3 Days
- 1-3 Days
- 24-48 Hours
- 24-48 Hours
- Up to 80 Hours
- 24-48 Hours
- 24-48 Hours
- –
- 12-24 Hours
- 12-24 Hours
- 48-72 Hours
- 1-3 Days
- 1-3 Days
- 48-72 Hours
- 24-48 Hours
- 1-3 Days
- 1-3 Days
- 1-3 Days
- 1-3 Days
- 48-72 Hours
- 1-2 Days
- 48-72 Hours
Weight | 0.25 lbs |
---|---|
Dimensions | 6 × 4 × 6 in |
Adulterants |
No |
Drugs Tested For | COT |
Number of Drugs Tested | 1 |