Monkeypox Virus Real-time PCR (RTPCR) test/80 testsMV Reaction tube (80)/ MV Positive Control(1)/MV Negative Control (1)

  • Case of 80
  • SKU: A408003
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The kit is based on RTPCR technology and PCR amplification in one-step. The assay amplifies the conserved gene of the Monkeypox viral DNA in blood, urine, skin and mucous membrane damage, and respiratory (nasopharyngeal, oropharyngeal, naso-oropharyngeal) samples where this targeted region is amplified and detected by designed primers and fluorescent probes. An additional primer/probe set is used to detect the human gene (RNase P gene) for Internal Control (IC). The IC is used to ensure proper execution of the assay by the end user, including sample collection, nucleic acid extraction and the PCR process.
 
The Monkeypox RTPCR kit is composed of MV Reaction tube, MV Positive Control and MV Negative Control.
 
  • The MV Reaction tube includes the Monkeypox virus DNA detection system, including the internal control. It contains primers and FAM-labeled probe specific for the conserved region gene of Monkeypox virus, HEX-labeled probe specific for the conserved region gene of Monkeypox virus, and CY5-labeled probe specific for internal control. And the MV Reaction tube also contains the Taq DNA polymerase for PCR amplification and uracil-N-glycosylase to prevent PCR amplicon carryover contamination.
  • The MV Positive Control contains armored DNA for the conserved region gene and internal control RNase P gene.
  • The MV Negative Control (no template control).

Features

  • One-step amplification
  • Targets conserved gene region
  • Includes human internal control

Performance

  • Three hundred (n=300) simulated samples were run using both the Anbio Monkeypox Virus RTPCR Kit and a competitor kit already on the market. The results of the comparison were that the
  • Anbio Monkeypox Virus RT-PCR Kit was both 100% for sensitivity and specificity for the group of samples tested.

 

   Contents

   Main Ingredients

Specification

48 Tests/ kit

80 Tests /kit

200 Tests/ kit

   MV Reaction tube

Primers, Probes, Mg2+, dNTPs, Taq DNA Polymerase,

Uracil-N-Glycosylase

8-tube strip×6

8-tube strip×10

8-tube strip×25

   MV Positive Control

Armored DNA for Monkeypox virus, internal control (RNase P gene)

1 tube × 1

1 tube × 1

1 tube ×2

   MV Negative Control

No template control

1000μL/tube ×1

1000μL/tube ×1

1000μL/tube ×2

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